Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

48_05_06

E-mail Print PDF

Malays. Appl. Biol. (2019) 48(5): 49–57

 

A COMPARATIVE STUDY OF THE BIOLOGICAL ACTIVITIES

OF DIFFERENT COMMERCIAL Stevia EXTRACTS FROM LOCAL

MARKET IN MALAYSIA


SUHAILI SHAMSI1*, FARHAH AMIRAH ZUKEFLLI1, AI LUAN LIM1 and

USWATUN HASANAH ZAIDAN2


1Laboratory for Animal Biochemistry and Biotechnology, Department of Biochemistry,

Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia,

43400 UPM, Serdang, Selangor, Malaysia

2Laboratory for Food and Microbiome Technology, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 28 August 2019, Published online 31 December 2019


ABSTRACT

An increased awareness on consuming healthy food and beverages worldwide has led to an upsurge of interest in functional food, which includes the use of natural sweetener, the Stevia rebaudiana Bertoni extract. Nevertheless, limited data has been available on the biological activities of commercial Stevia extract available in Malaysia. Hence, the present study aims to evaluate the biological activities of commercial Stevia extract from local market in Malaysia, by evaluating its total phenolic (TPC) and total flavonoid content (TFC), as well as its antioxidant and anti-diabetic activities. Three independent Stevia extracts (Samples A, B and C) sourced from local market in Malaysia were evaluated, in comparison to the freshly prepared Stevia extract. The results showed a significantly lower amount of TPC in commercial Stevia extracts when compared to the freshly prepared Stevia extract (7.077 mg GAE/100 g), with sample A containing the highest TPC (6.359 mg GAE/100 g), followed by sample C (1.496 mg GAE/100 g) and sample B (0.624 mg GAE/100 g). Similar trend was observed with TFC, with sample A containing 0.937 mg QE/100 g, followed by sample C (0.264 mg QE/100 g) and sample B (0.029 mg QE/ 100 g) as compared to the freshly prepared Stevia extract (1.684 mg QE/100 g). Sample A showed the highest antioxidant activity (DPPH: 48.84%, FRAP: 2.589 ?mole Fe2+/g, ABTS: 28.48%), albeit lower to the freshly prepared Stevia extract. The commercial samples showed a higher ?-amylase inhibition activity compared to the freshly prepared Stevia extract, but no inhibition was observed in the ?-glucosidase activity. Fundamentally, the results highlight the biological activities of Stevia extract for functional food applications, but caution has to be exercised as all three commercial extracts have significantly different biological activities.


Key words: Stevia rebaudiana, commercial stevia extract, biological activity, antioxidant activity, ?-amylase, ?-glucosidase

 

Latest MABJ Issue

Vol 49(2) July 2020

coverphoto

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Elsevier, Mycite, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.