Malaysian Applied Biology Journal

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Malays. Appl. Biol. (2019) 48(5): 1–10




OF CATFISH (Pangasius hypopthalmus)


1Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Diponegoro University,

Jl. Prof. Soedarto, SH, Tembalang, Semarang, Central Java 50275 Indonesia

2Department of Fishery Product Technology, Faculty of Fisheries and Marine Science,

Brawijaya University, Jl. Veteran, Malang, East Java 65145, Indonesia

3Bio-Seafood Research Unit, Brawijaya University, Jl. Veteran, Malang, East Java, Indonesia

4Halal Thoyib Science Center, Brawijaya University, Jl. Veteran, Malang, East Java, Indonesia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Accepted 15 August 2019, Published online 31 December 2019


This study aims to evaluate the effect of papain enzyme diet on growth performance of Catfish (Pangasius hypopthalmus) through feed efficiency and growth parameters. The research employed a completely randomised design with four treatments and three replicates. Catfish fingerlings with an average weight of 2.23 g/fish were used as samples. The experimental diets were prepared to be isonitrogenous (31%) and isocaloric (252.06 kcal/g). Papain enzyme was added to the feed within various doses; A (0 g/kg feed), B (2 g/kg feed), C (4 g/kg feed), D (6 g/kg feed) and E (8 g/kg feed). The study parameters included apparent digestibility coefficient of protein (ADCP), relative growth rate (RGR), the efficiency of feed utilisation (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), survival rate (SR) and water quality. The results of the study show that the addition of papain enzyme significantly affected ADCp, RGR, EFU, FCR, and PER, but not the SR of catfish. Based on regression analysis, the optimum dose of ADCP and RGR were 4.05 g/kg, RGR was 4 g/kg feed, respectively. The EFU had an optimum dose of 3.93 g/kg feed, meantime the FCR and PER had an optimum dose of 4 g/kg feed each. In conclusion, the diet of papain in the amount of 4 g/kg feed increased the growth performance and nutrient utilisation of catfish (Pangasius hypopthalmus).

Key words: Diet, papain enzyme, catfish (Pangasius hypothalamus), growth performance


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