Malaysian Applied Biology Journal

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48_04_16

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Malays. Appl. Biol. (2019) 48(4): 121–127

 

IN VITRO ALPHA-GLUCOSIDASE AND ALPHA-AMYLASE

ENZYME-INHIBITED ACTIVITIES BY WATER-LILY

(Nymphaea genus) EXTRACTS


KORAWINWICH BOONPISUTTINANT1*, SUPANIDA WINITCHAI2, PURIN AKKARAKULTRON3

and SARINPORN UDOMPONG1*


1Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi,

Pathum Thani, 12130, Thailand

2Kasetsart Agricultural and Agro-industrial Product Improvement Institute,

Kasetsart University, Bangkok 10300, Thailand

3Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi,

Pathum Thani, 12130, Thailand

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ; This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 14 October 2019, Published online 30 November 2019

 

ABSTRACT

Chalong Kwan (C), Khao Mongkol (K) and Chompoo Mameaw (P) are the water-lily in the family of Nymphaeaceae which were planted in Thailand. In this study, leaves (1), flowers (2), tuber (3), pollen (4), branches (5) of the three water-lily were boiled with distilled water (W) and macerated on 95% ethanol (E). The extracts from water-lily including C3-E, P1-E, P3-E, C1-W, P1-W, P3-W, and P5-W were showed the highest level of free radical scavenging activities measured by (DPPH) assay (SC50 range of 0.003-0.007), which is more than L-ascorbic acid (SC50 of 0.013 ± 0.0001 mg/mL) about four times. The effect of anti-diabetic was determined inhibitory activities of ?-glucosidase and ?-amylase by enzymatic assay. P4-E extract was the highest exhibited the ?-amylase activities (AC50 of 0.02 ± 0.001 mg/mL) with higher than acarbose for ten times (AC50 of 0.23 ± 0.14 mg/mL). Whereas, the C2-E, C3-E, and C1-W extracts inhibited the activity of ?-glucosidase (GC50 range of 0.08-0.11), which higher than acarbose for 6 times (GC50 of 0.63 ± 0.04 mg/mL). The ethanol extracts from Chompoo Mameaw (P1-E and P3-E) which demonstrated the highest antioxidant and anti-diabetic activity, might be possible for further development to natural products for diabetic patients.


Key words: Water-lily, Nymphaeaceae, anti-diabetic, free radical scavenging

 

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Vol 48(4) November 2019

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