Malaysian Applied Biology Journal

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48_03_17

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Malays. Appl. Biol. (2019) 48(3): 149–156

 

PRODUCTION AND TOXICITY EVALUATION OF RHAMNOLIPIDS

PRODUCED BY Pseudomonas STRAINS ON L6 AND HepG2 CELLS


ARULARASU MUTHALIAR TAMOTHRAN1, VIGNESWARI SEVAKUMARAN2,3

and KESAVEN BHUBALAN1,3,4*


1School of Marine and Environmental Sciences, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

2School of Fundamental Sciences, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

3Institute Marine Biotechnology (IMB), Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

4Malaysian Institute of Pharmaceuticals and Nutraceuticals (IPharm), NIBM,

11700 Gelugor, Pulau Pinang, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 22 May 2019, Published online 30 June 2019


ABSTRACT

Biosurfactant rhamnolipid (RL) production using renewable resource is gaining attraction for commercial application. In this study, RL produced from three different strains of Pseudomonas using glycerol as a carbon source was used to evaluate toxicity towards rat skeletal muscle (L6) and liver cancer (HepG2) cells. In the present study, Pseudomonas aeruginosa PAO1 produced the highest concentration of RL (1.53 ± 0.28 g/L) and able to reduce the surface tension (ST) value of water the lowest (29.1 ± 0.5 mN/m). Toxicity evaluation using MTT assay indicated that RL produced does not have a cytotoxic effect towards both cell lines except where 50% inhibition concentration (IC50) was detected for HepG2 only at high concentration (100 ?g/mL) for RL produced by P. aeruginosa PAO1. The RL produced by strains in this study is nontoxic with good ST reducing ability that has potential applications in food, cosmetics and pharmaceutical sector.


Key words: Pseudomonas, biosurfactant, rhamnolipid, cytotoxicity, marine bacteria

 

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