Malaysian Applied Biology Journal

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48_03_10

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Malays. Appl. Biol. (2019) 48(3): 85–93

 

COMPARISON OF GENETIC DIVERSITY OF LEPTIN GENE

BETWEEN WILD GOAT AND DOMESTIC GOAT

BREEDS IN IRAN


NARGES ESMAEILI ALIABAD VAZIR1*, ALI ESMAEILIZADEH KOSHKOOIEH1,

AHMAD AYTOLAHI MEHRJERDI1, MOHAMMADREZA MOHAMMADABADI1,

OLENA IVANIVNA BABENKO2, MARYNA VITALIIVNA BUSHTRUK2,

SERHII VASYLIOVYCH TKACHENKO2, RUSLANA VOLODYMYRIVNA STAVETSKA2

and NATALIIA IHORIVNA KLOPENKO2


1Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran

2Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 13 May 2019, Published online 30 June 2019


ABSTRACT

Leptin is a polypeptide which is mostly secreted by the white adipose tissue and a little by gastrointestinal tract and placenta, plays an important role for controlling body weight, feed intake, immunity, milk production, and reproduction. The aim of this study was to investigate the exon 2 of the leptin gene polymorphism in Iranian wild and domestic goats using PCRRFLP. Blood samples were collected from 14 wild and domestic goat breeds including (Cashmere Abadeh, Torki-Ghahghaei, Naeini, Robati, Nadoushani, Adani, Shahrbabaki, Birjandi, China goat, Sannen, Pakistani, Raeini cashmere, Najdi, and Wild goat) and then genomic DNA was extracted. A 152 bp fragment from exon 2 of the leptin gene was amplified. PCR products were digested with Hinf I restriction enzymes and was separated and visualized on the agarose gels. From possible three genotypes (TT, TC, and CC), only two genotypes TT and CC were observed in 14 studied domestic and wild populations with the genotype frequency of 95% and 5% respectively. The number of observed alleles, number of effective alleles, Nei’s Index and Shanon’s Index were 2, 1.10, 0.10 and 0.20 respectively. The studied populations were not found to be in Hardy-Weinberg equilibrium. Our investigation demonstrated that TT genotype and T allele had a very high frequency (0.95) in studied goats. Hence, it can be concluded that this finding can provide the basis for selection when considering evolution and differentiation among breeds, however, further studies should be carried out on a larger population of different domestic and wild breeds to verify the final conclusions.

Key words: Diversity, domestic goat, leptin gene, PCR-RFLP, wild goat

 

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