Malaysian Applied Biology Journal

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48_03_09

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Malays. Appl. Biol. (2019) 48(3): 77–83

 

ENZYME INHIBITORY ACTIVITIES OF MARINE SPONGES

AGAINST CHOLINESTERASE AND 5?-REDUCTASE


SUCIATI1,2*, KARMA RABGAY3, YUNDA FACHRUNNIZA3, TONGCHAI SAESONG3,

TRI ARYONO HADI4, TUTIK SRI WAHYUNI1,2, ATY WIDYAWARUYANTI1,2

and KORNKANOK INGKANINAN3


1Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy,

Universitas Airlangga, Jl. Dharmawangsa Dalam, Surabaya 60286, East Java, Indonesia

2Institute of Tropical Diseases, Universitas Airlangga, Jl. Mulyorejo,

Surabaya 60115, East Java, Indonesia

3Bioscreening Unit, Department of Pharmaceutical Chemistry and Pharmacognosy,

Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry,

Naresuan University, Phitsanulok, Thailand

4Division of Marine Resources, Research Center for Oceanography,

Indonesian Institute of Sciences, Jl. Pasir Putih 1, Ancol Timur, Jakarta 14430, Indonesia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 10 April 2019, Published online 30 June 2019

 

ABSTRACT

Marine sponges have been the source of various metabolites with potent biological activities. In this study fifteen methanolic extracts of marine sponges, collected off the coast of Tabuhan Island, Banyuwangi, East Java, Indonesia were evaluated in relation to their cholinesterase and 5?-reductase inhibitory activities. The results revealed that the extract of Petrosia sp. inhibited the 5?-reductase enzyme at 100 ?g/mL, with 61.21% inhibition, which is slightly lower than the positive control, finasteride, of 76.70%. The results of the cholinesterase inhibitory screening showed that three marine sponges namely, Callyspongia sp., Niphates olemda, and Agelas nakamurai presented notable cholinesterase inhibitory activities. The highest potency was found in A. nakamurai, with an IC50 value of 1.05 ?g/mL. All three samples inhibited both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), however, the extract of N. olemda showed a higher inhibition against AChE compared to BuChE. The chemistry of the Callyspongia sp., N. olemda and A. nakamurai were investigated using thin layer chromatography and 1H NMR methods. The results suggested the presence of terpenes and alkaloids in the samples. Further study is needed to determine the metabolite responsible for cholinesterase inhibitory activity.

Key words: Marine sponge, acetylcholinesterase inhibitor, 5?-reductase inhibitor

 

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