Malaysian Applied Biology Journal

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48_02_08

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Malays. Appl. Biol. (2019) 48(2): 55–62

 

ESSENTIAL OIL CONSTITUENTS ASSESSMENT AND

ANTIOXIDANT PROFILES OF Stevia rebaudiana

BERTONI LEAVES FROM MALAYSIA


NUR IZZATI, M.Z.1, USWATUN HASANAH, Z.1,2*, SITI SALWA, A.G.1,3,

SUHAILI, S.2 and MOHD. IZUAN EFFENDI, H.3


1Halal Products Research Institute,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

3Department of Agriculture Technology, Faculty of Agriculture,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 2 May 2019, Published online 31 May 2019


ABSTRACT

Since the 15th century, essential oils (EO) were having a strong interest as therapeutic agents and recently EO has been widely used in pharmaceutical, cosmetic, food and agricultural industries. The previous study showed that EOs from Stevia rebaudiana Bertoni (SrB) possess high antioxidant, anti-inflammatory, and antimicrobial properties. In this study, the chemical constituents of EO from SrB leaves which grown in Selangor, Malaysia were obtained by ethanol Soxhlet extraction. Total polyphenolic content (TPC), total flavonoid content (TFC), and chemical composition using a gas chromatography-mass selective detector (GC-MSD) analyses of Stevia rebaudiana Bertoni essential oil (SrB EO) were carried out. The TPC and TFC of SrB EO were 14.44±0.63 mg gallic acid equivalent (GAE) /g sample extract and 32.19±1.38 mg quercetin equivalent (QE)/g sample extract, respectively. Based on GC-MSD analyses, one hundred peaks were obtained and 28 compounds were identified where lupeyl acetate occupied the highest percentage with 14.25% of the chemical constituents. The antioxidant activities of SrB EO were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assays. The IC50 of SrB EO using DPPH and ABTS were 71.36±1.57 ?g/mL Trolox equivalent (TE) and 8.88×102±0.16 ?g/mL TE, respectively while the FRAP activity was 9.55±0.26 mg/g TE. These results show the promising antioxidant properties of SrB EO.


Key words: Essential oils; Stevia rebaudiana Bertoni; antioxidant activities; GC-MS

 

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