Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

48_02_04

E-mail Print PDF

Malays. Appl. Biol. (2019) 48(2): 27–31

 

OPTIMIZATION OF Nigella sativa OIL-BASED FERULATE ESTER

SYNTHESIS USING RESPONSE SURFACE METHODOLOGY


FARIHIN AZHARI1, USWATUN HASANAH ZAIDAN1,2*, SYAHIDA AHMAD2 and

SITI SALWA ABD. GANI3


1Halal Products Research Institute, Universiti Putra Malaysia, Putra Infoport,

43400 UPM, Serdang, Selangor, Malaysia

2Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

3Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia,

43400 UPM, Serdang, Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 7 May 2019, Published online 31 May 2019


ABSTRACT

Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67 mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained was 49.87% which is acceptable with the predicted value of 48.82%.

Key words: Nigella sativa oil, enzymatic transesterification, plant oil-based ferulate ester, optimization, response surface methodology (RSM)

 

Latest MABJ Issue

Vol 48(3) June 2019

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Myjurnal, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.