Malaysian Applied Biology Journal

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48_01_10

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Malays. Appl. Biol. (2019) 48(1): 67–72

 

BACTERIA ISOLATION FROM SKIN, GILLS AND WATER OF

Oreochromis sp. (RED TILAPIA) WHEN TREATED WITH

Eisenia foetida (OLIGOCHAETE), Pleurotus sajor-caju (OYSTER

MUSHROOM), AND Nepenthes gracilis (PITCHER PLANT)


NUR SYUHADA BINTI ROSLI, ELVIE JOHN BAPTIST and NOR OMAIMA BINTI HARUN*


School of Fundamental Science, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 23 January 2019, Published online 20 March 2019

 

ABSTRACT

Aquaculture farmers are focused on large intensive farming to fulfill the demand of protein source, thus increasing the probability of bacteria-related disease outbreak. Therefore, an alternative fish diet has to be introduced to reduce bacteriarelated infection while increasing the production. The aim of this study was to determine the microbial community on Oreochromis sp. when treated with modified diets; commercial pellet added with oligochaete (Eisenia foetida), oyster mushroom (Pleurotus sajor-caju), pitcher plant (Nepenthes gracilis) and combination of all ingredients with proportion of (90:10) and (70:10:10:10). One hundred ninety bacterial colonies from 11 species were identified using biochemical tests: Gram stain, Oxidase test, Catalase test, KOH String test, Carbohydrate (Glucose, Lactose and Sucrose) test, Indole test, Methyl Red Test and Voges-Proskauer Test (MRVP test), Citrate test and bacteria growth tested on Mannitol salt agar. Oreochromis sp. also secretes mucus that consists of protein to protect fish from pathogens with various environment conditions. Therefore, this study can be used to increase a better understanding of modified organic diet with higher protein content of microbial community in lower vertebrates.

Key words: Bacteria identification, Eisenia foetida, feed utilization, Nepenthes gracilis, Pleurotus sajor-caju, protein content

 

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