Malaysian Applied Biology Journal

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48_01_06

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Malays. Appl. Biol. (2019) 48(1): 35–41

 

DEGRADATION OF DIESEL OIL BY SOIL BACTERIA IN SHAKE

FLASK SYSTEM USING FOOD WASTE AMENDMENTS


GAN SIK ZE1, NORAZNAWATI ISMAIL2 and FAZILAH ARIFFIN1*


1School of Fundamental Science, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

2Institute Marine Biotechnology, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 4 January 2019, Published online 20 March 2019

 

ABSTRACT

Diesel oil contains compounds that can cause harm to humans and the environment. Hence, biodegradation method is an alternative way to reduce the pollution caused by diesel oil. The aim of this study is to determine the diesel oil degradation by soil bacteria amended with food wastes in the flasks system. It also aims to determine the food wastes such as sugarcane bagasse and fishbone to enhance the biodegradation of diesel oil. The degradation analysis was performed in an enrichment culture flask containing soil, diesel oil with the addition of food waste. The degradation analysis was carried out for 42 days at 30°C at 150 rpm. The bacteria was isolated and identified based on colony morphology and biochemical tests. Five potential diesel oil-degrading bacteria were preliminary identified as Pseudomonas fluorescens, Pseudomonas aeruginosa, Klebsiella species, Shewanella putrefaciens and Bacillus cereus. Diesel oil degradation compound was analyzed using Gas Chromatography - Mass Spectrometry. Four compounds namely styrene, ethanol, 2-butoxy, benzene, 1-ethyl-2, 3-dimethyl and benzene 1-ethyl-2, 3-dimethyl showed degradation by bacteria amended with food wastes. The results of this study demonstrate the potential use of food wastes such as sugarcane bagasse and fish bone as substrates for enhancing the remediation of hydrocarbon contaminated soil.

Key words: Degradation, diesel oil, soil bacteria, shake flask

 

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