Malaysian Applied Biology Journal

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48_01_05

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Malays. Appl. Biol. (2019) 48(1): 27–33

 

MORPHOLOGICAL CHANGES AND DNA DAMAGE IN

Chlorella vulgaris (UMT-M1) INDUCED BY Hg2+


HAZLINA, A.Z.*, DEVANTHIRAN, L. and FATIMAH, H.


School of Fundamental Science, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu Darul Iman, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 26 January 2019, Published online 20 March 2019

 

ABSTRACT

This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 ) of Hg2+ on the growth of C. vulgaris obtained from the dose-response curve was 0.72 mg/L. Under SEM, it was observed that Hg2+ treated cells become smaller in size (i.e. 2.1±0.1 ?m) compared to normal cells (i.e. 3.2±0.1 ?m). The morphology of cells changed from an intact microalgal cells with smooth spherical surface to a slightly roughened surface and shrivelled shape. Apoptotic bodies were also observed under 0.001, 0.01 and 0.1 mg/L of Hg2+ but not in 1.0 mg/L Hg2+. These results indicate that Hg2+ may induce apoptotic cell death at low concentrations but not at the highest concentration of 1.0 mg/L. The highest percentage of cells with comet level of 4 was observed in 1.0 mg/L Hg2+. However, Hg2+ is genotoxic to the microalga even at low concentration. In conclusion, Hg2+ can exert its toxic effects on C. vulgaris by changing the microalgal morphology, damaging the microalgal DNA and inducing cell death in the microalgal cells using different pathways at different concentrations.

Key words: Microalgae, heavy metals, comet assay, genotoxic effect, cell death

 

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