Malaysian Applied Biology Journal

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47_06_12

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Malays. Appl. Biol. (2018) 47(6): 97-104

 

GENETIC VARIATION, DIVERSITY AND MOLECULAR

PHYLOGENETIC OF HIGHER GROUP TERMITE

Macrotermes carbonarious Hagen (BLATTODEA: TERMITIDAE)

 

ABDUL HAFIZ AB MAJID1*, ENG YI SHEN1,2, CHONG YEAK HENG1,2 and LIEW CHIN FOONG1,2


1Household & Structural Urban Entomology Laboratory, School of Biological Sciences,

Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia

2Department of Biological Science, Universiti Tunku Abdul Rahman, Kampar, Perak, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 10 December 2018, Published online 31 December 2018

 

ABSTRACT

Limited studies pertaining to evolutionary history and intraspecific diversity of Macrotermes genus have been carried out by researchers. Such studies are vital as from phylogeny we can understand the origin of species in a particular region. Thus, in this study, we investigated the phylogenetic relationships and genetic diversity among the population of Macrotermes carbonarius around USM campus. Seventeen Macrotermes carbonarius colonies were used to conduct a molecular genotyping study and the mitochondrial (COII) gene was used as marker. The data obtained were analyzed using T-Coffee, Clustal X, Mega 7.0 and DnaSP software programs. Our results show one main monophyletic clade that consists of two groups: HP1 and HP2. The samples from Stadium Hoki were classified in HP2, differing from the other populations studies by one singleton base. Our HP2 shows some similarity with some sequence from Pasoh Forest Reserved Malaysia, suggesting a state diversity connection. The disparity index suggests that all the samples are homogeneous populations, supported by the low value of mean pairwise distance between the samples. This study will serve as a platform for scientific community to enhance the knowledge on the phylogeny and genetic diversity of Macrotermes genus in South East Asia region.

Key words: Macrotermes carbonarius, phylogeny, genetic diversity, haplotype, mitochondrial COII gene

 

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