Malaysian Applied Biology Journal

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47_06_05

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Malays. Appl. Biol. (2018) 47(6): 41–45

 

MOLECULAR AND PHYLOGENETIC IDENTIFICATION OF

MARINE MICROALGAE INFERRED BY 18S RDNA GENE


NAZIA ABDUL KADAR1, SITTI RAEHANAH MUHAMAD SHALEH1* and EMMA SU ALI2


1Borneo Marine Research Institute, Universiti Malaysia Sabah, Jalan UMS,

88400 Kota Kinabalu, Sabah, Malaysia

2Faculty of Engineering, Universiti Malaysia Sabah, Jalan UMS,

88400 Kota Kinabalu, Sabah, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 22 September 2018, Published online 31 December 2018

 

ABSTRACT

Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels.

Key words: 18S rDNA, microalgae, Chaetoceros, Thalassiosira, Nannochloropsis, Isochrysis

 

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