Malaysian Applied Biology Journal

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47_05_35

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Malays. Appl. Biol. (2018) 47(5): 283–288

 

INCORPORATION OF SILVER NANOPARTICLES (AgNPs) WITH

ENDOPHYTES CELL-FREE SECONDARY METABOLITES

ENHANCES ANTIMICROBIAL ACTIVITY


AHMAD SYAIRAZIE IBRAHIM FIKRI1, IRMAN ABDUL RAHMAN2, AINON HAMZAH1,

SITI NOOR ADNALIZAWATI ADNAN3 and NOREFRINA SHAFINAZ MD NOR1*


1School of Biosciences and Biotechnology, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

2School of Applied Physics, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

3Faculty of Dentistry, Universiti Sains Islam Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 30 October 2018, Published online 30 November 2018


ABSTRACT

In our previous study, we isolated eight bacterial endophytes with positive antimicrobial activity out of 82 isolates from five different places in Pulau Langkawi. The antimicrobial activity of the endophytes secondary metabolite although moderate, was further explored for the prospect to be enhanced by incorporating to silver nitrates. Only two isolates were able to synthesise silver nanoparticles namely Bacillus subtilis (B7b) and Pseudomonas resinovorans (B2c) within 72 hours of incubation at room temperature via extracellular method. Antimicrobial assay against Staphylococcus aureus, methicilin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermis, Escherichia coli and Pseudomonas aeruginosa was performed. Synthesised silver nanoparticles (AgNPs) showed excellent inhibitory effect against both Gram positive and Gram negative bacteria but secondary metabolites without AgNPs showed positive activity only against Gram positive bacteria. The results suggested that incorporation of AgNPs was able to enhance antimicrobial activity of secondary metabolites of B7b and B2c against test bacteria.

Key words: Helicobacter pylori, genotype, gastric biopsies, cockroaches

 

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