Malaysian Applied Biology Journal

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47_05_32

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Malays. Appl. Biol. (2018) 47(5): 261–267

 

Paenibacillus alvei (AN5) AND GRAMICIDIN A ANTIMICROBIAL

PEPTIDE ACTIVITIES


SAHLIL MIRAZ MOHAMED RAFIE1, DARMAN NORDIN1,2* and NURINA ANUAR1,2


1Research Centre for Sustainable Process Technology (CESPRO),

Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia,

43600 UKM Bangi, Selangor

2Chemical Engineering Programme, Faculty of Engineering and Built Environment,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 2 October 2018, Published online 30 November 2018


ABSTRACT

Antibacterial resistance is a major problem faced in public health. Antimicrobial peptides (AMPs) has gained attention as potential for antiinfectives due to their wide broad activity. AMPs are part of universal defense system in almost all living organism. In this study, antimicrobial activity of AMP crude extract from Paenibacillus alvei AN5 (crude AN5) and commercial AMP (Gramicidin A, gA) were tested against Gram positive bacteria, Staphylococcus aureus and Gram negative bacteria, Escherichia coli using broth dilution method. The results express in turbidity measurement showed reduction of S. aureus growth by 64% with treatment of gA and 50% with treatment of crude AN5. For E. coli, the percentages of reduction are 49% and 43%. For the effect of different concentrations of AMP, S. aureus was inhibited at 10% v/v of gA, 100% for crude AN5 and E. coli 50% gA, 100% crude AN5. Crude AN5 and gA were capabale of inhibiting both Gram negative and positive bacteria.

Key words: Antimicrobial activity, gramicidin A, P. alvei AN5

 

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