Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

47_05_12

E-mail Print PDF

Malays. Appl. Biol. (2018) 47(5): 89–100

 

IDENTIFICATION OF RESTRICTION-MODIFICATION SYSTEMS

IN Clostridium butyricum USING BIOINFORMATIC TOOLS


ROZIEFFA ROSLAN1, PEER MOHAMED ABDUL1,2* and JAMALIAH MD JAHIM1,2


1Research Centre for Sustainable Process Technology (CESPRO),

Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia,

43600 UKM Bangi, Selangor, Malaysia

2Chemical Engineering Programme, Faculty of Engineering and Built Environment,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 17 October 2018, Published online 30 November 2018


ABSTRACT

Genetic manipulation is an extremely robust method to enhance the yield of major fermentation product. Nevertheless, restriction-modification (RM) system is the main barrier to genetic manipulation, as it prevents foreign DNA transfer into the host. In order to investigate the presence of restriction enzymes homolog in Clostridium butyricum, a comparative analysis was performed on restriction enzyme sequences from REBASE collection against genome and amino acid sequences of C. butyricum using BLAST program. Multiple restriction enzymes homolog with more than 75% query cover were recognised in the genomes. Based on sequence analysis of five strains, Type II RM system is the most abundant (63.16%), Type IV is the second most abundant (21.05%), followed by Type III (10.53%) and Type I (5.26%). By utilising whole genome sequences and restriction enzyme database, we can comprehend the biological aspect of strain-specific RM systems to develop better strategies and techniques in order to overcome the restriction barrier in genetic manipulation.

Key words: Genetic engineering, genetic manipulation, restriction enzyme, Clostridium, evolution

 

Latest MABJ Issue

Vol 48(2) May 2019

Cover Photo

Table of content

Latest news!

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.