Malaysian Applied Biology Journal

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47_05_10

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Malays. Appl. Biol. (2018) 47(5): 71–80

 

DECIPHERING THE PAN-GENOME OF Ganoderma sp. TO DEPICT

POTENTIAL GENOMIC COMPONENTS THAT CONTRIBUTE

TO Ganoderma boninense PATHOGENICITY


SUHAILA SULAIMAN*, NUR SYAMIMI YUSOFF, TAN JOON SHEONG and LEE YANG PING


FGV Research & Development Sdn. Bhd.,

FGV Innovation Centre (Biotechnology), PT. 23417, Lengkuk Teknologi,

71760, Bandar Enstek, Negeri Sembilan

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 19 October 2018, Published online 30 November 2018


ABSTRACT

The soil-borne fungus, Ganoderma boninense is notorious as the cause of basal stem rot (BSR) disease in oil palm that results in severe economic losses. Due to insufficient functional information of its genome, mechanism underlying the infection is still enigmatic. Despite the constraint, here we report the pan-genome of eight draft genomes of Ganoderma sp. that delineated into 35,121 orthologous genes (OGs), consisting 4,898 genes in core genome and 30,223 genes in accessory genome, whilst the latter encompasses of 1,905 species-specific genes. Further genome-wide comparative analysis discovers 607 genes in pathogenic fungi G. boninense but not in its related non-pathogenic Ganoderma sp., suggesting their potential role in G. boninense pathogenicity towards its host. By implementing various bioinformatics tools and public databases, the incorporated functional enrichment of the candidate genes leads to inference of their role during host-pathogen interaction. This enables us to narrow down the entire gene catalogue in Ganoderma sp. for experimental verification in the laboratory. It is undeniable that the harnessing of Ganoderma sp. pan-genome data will conform to a more structured downstream analysis in understanding the role of G. boninense as the key agent of BSR in oil palm.

Key words: Pan-genome, Ganoderma boninense, pathogenicity, basal stem rot

 

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