Malaysian Applied Biology Journal

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47_05_09

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Malays. Appl. Biol. (2018) 47(5): 63–69

 

CHARACTERISATION AND PATHOGENICITY OF Fusarium

SPECIES ISOLATED FROM LUFFA (Luffa acutangula L. Roxb.)


ASMA, A.1, SHAMARINA, S.1, NOOR BAITY, S.2 and NUR AIN IZZATI, M.Z.1*


1Department of Biology, Faculty of Science, Universiti Putra Malaysia,

43400 Serdang, Selangor, Malaysia

2Department of Molecular and Cell Biology, Faculty of Biotechnology and Biomolecular Science,

Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 24 October 2018, Published online 30 November 2018


ABSTRACT

Fusarium is a soil-borne fungus, comprises most of the pathogenic species ubiquitously found in subterranean, aerial parts of plants, plants debris, and distinct organic substrates. The fungus was well recognised to cause diseases in plant such as root rot and vascular wilt in a broad range of horticulture crops including cucurbits. In this study, 19 isolates were retrieved from infected luffa for confirmation on pathogenicity using Koch’s Postulates. All isolates were identified and classified based on translation elongation factor (tef1-?) and beta-tubulin (?-tub) gene sequence analyses. The Fusarium isolates virulence was then verified. Luffa seeds were soaked into 2×106 spore/ml of conidial suspension for 12 hours before sowing. As control, seeds were inoculated into sterile distilled water. After 30 days post-inoculation (dpi), most virulent isolates were Fusarium solani D2499L and Fusarium proliferatum B1777L with disease severity of 66.75%, respectively. Four isolates identified as Fusarium incarnatum presented non-virulence with no visible wilt symptoms. This study revealed Fusarium species diversity and pathogenicity for better management of disease in cucurbit plants.

Key words: Fusarium, cucurbits, virulence, pathogenic species, disease severity

 

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