Malaysian Applied Biology Journal

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47_03_15

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Malays. Appl. Biol. (2018) 47(3): 121–127

 

POTENCY OF A MARINE SPONGE FROM THE GENUS Stylissa

AS ANTI HEPATITIS C AGENT

 

SUCIATI1,2*, ACHMAD FAUZI1, MYRNA ADIANTI2,3 and ACHMAD FUAD HAFID1,2


1Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga,

Jl. Dharmawangsa Dalam Surabaya 60286, East Java, Indonesia

2Institute of Tropical Diseases, Universitas Airlangga, Jl. Mulyorejo,

Surabaya 60115, East Java, Indonesia

3Traditional medicine Study Program, Department of Health, Faculty of Vocational Study,

Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo 47, Surabaya 60131, East Java, Indonesia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Accepted 6 May 2018, Published online 30 June 2018


ABSTRACT

Marine sponges have shown potential as a source of many bioactive metabolites. Our previous study on Indonesian marine sponges indicated that this organism possessed promising antiviral activity. The aim of this study was to screen antiviral activity of marine sponge extract from the genus Stylissa and its fractions against hepatitis C virus (HCV). Identification of metabolites in the active fractions was also carried out as well as investigation of the mode of action of the extract. The results showed that the extract inhibited the growth of HCV at IC50 of 4.4 ?g/mL. The time of addition experiment revealed that the extract act at the post entry step. Bioassay-guided fractionations revealed two active fractions, namely fractions SF7 and SF8 which gave 99.3% and 86.4% inhibition, respectively, at a concentration of 50 ?g/mL. 1H NMR and GC-MS analyses indicated the presence of a mixture of saturated and unsaturated fatty acids in the active fractions.

Key words: Marine sponge, Stylissa, hepatitis C virus, fatty acids

 

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