Malaysian Applied Biology Journal

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47_03_02

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Malays. Appl. Biol. (2018) 47(3): 13–21

 

DNA CONTENT AND GENOME SIZE OF HIGHLY VALUED

MALAYSIAN AGARWOOD, Aquilaria malaccensis LAMK

 

SITI SUHAILA, A.R.1,3*, MOHD. SALEH, N.2, NORWATI, M.1, MAHANI, M.C.3,

NAMASIVAYAM, P.4 and KANDASAMY, K.I.5


1Forest Research Institute Malaysia (FRIM), 52109, Kepong, Selangor, Malaysia

2Agriculture Business Institute (ABI), 43400 Serdang, Selangor, Malaysia

3Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences,

Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia

4School of Environmental and Natural Resource Sciences, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor, Malaysia

5Malaysian Bioeconomy Sdn. Bhd., Level 23, Menara Atlan, 161B Jalan Ampang,

50450, Kuala Lumpur, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Accepted 27 November 2017, Published online 30 June 2018


ABSTRACT

Flow cytometry analysis of propidium iodide (PI) stained nuclei isolated from leaf tissues were used to estimate the DNA content and genome size of Aquilaria malaccensis Lamk. Samples of in vitro regenerated (tissue culture-derived plantlets) and ex vitro (wild seedlings) were evaluated. The results revealed that there was no intra-specific variation in A. malaccensis DNA content (1.84 pg 2C-1) and genome sizes (899 Mbp) between in vitro and ex vitro samples, suggesting that even after numerous sub-culturing and long-term culture periods, the species was able to retain stable genome size. On the other hand, interspecific variations were determined between A. malaccensis and five other Aquilaria species in terms of DNA content (1.84 to 1.92 pg 2C-1) and genome size (894.65 to 938.88 Mbp). This study provides valuable information that will facilitate genome sequencing and subsequent molecular studies of this economically important medicinal plant. Also, as evidence that micropropagation techniques have the potential to be used as a source of planting stock, along with seeds, for restoring locally threatened A. malaccensis wild populations and for commercial cultivation to supply the growing market demand.

Key words: Aquilaria, flow cytometer, DNA content, genome size

 

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