Malaysian Applied Biology Journal

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46_04_20

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Malays. Appl. Biol. (2017) 46(4): 169–174

A PRELIMINARY PROTEOMIC-BASED STUDY OF WILD MUD

CRAB (Scylla olivacea) RESPONSES TOWARDS THE

SURROUNDINGS OF SETIU WETLAND LAGOON,

TERENGGANU

 

NUR SYAFINAZ MOHD RAZALI1, FATHIN NOOR AZIERA MOHD UBADILLAH1,

WAN BAYANI WAN OMAR2, NURUL HUDA ABDUL KADIR1

and NAKISAH MAT AMIN1*

 

1School of Fundamental Sciences, Universiti Malaysia Terengganu,

21030 Kuala Nerus, Terengganu, Malaysia

2School of Marine & Environmental Science, Universiti Malaysia Terengganu,

21030 Kuala Nerus Terengganu, Malaysia


*Email: nakisah@umt.edu.my

 

Accepted 16 November 2017, Published online 31 December 2017

 

ABSTRACT

Scylla olivacea is one of the mud crabs species that is commonly found at Setiu Wetland, Terengganu. It serves as one of the income generating activities to the local community. Currently, the productivity of these mud crabs in the natural habitat is declined due to various factors. To understand the problems, water, sediment and tissues of mud crabs from the area were collected and analyzed using physico-chemical and proteomic analyses (1 dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE) and 2 dimensional isoelectric focusing (2D IEF)). Based on the results, water collected from Setiu Wetland showed salinity (ppt) of 16.3 ± 9.51, conductivity (?S/m) of 28.73 ± 15.78, temperature (°C) of 28.43 ± 0.57; and pH of 5.79 ± 1.55. Moreover, heavy metal level in the water (?g/L) and sediment (?g/g) respectively, revealed Zn: 13.40 ± 7.50 and 17.93 ± 2.29, Cu: 81.28 ± 20.70, 4.78 ± 0.78, Ni: 6.92 ± 0.24 and 5.83 ± 0.73, Pb: 2.25 ± 0.54 and 4.77 ± 0.52. Proteins of mud crabs’ tissues on 1D-SDS-PAGE demonstrated separation of protein bonds between 8 and 16. Meanwhile, the protein separation using 2D-IEF gels showed 61 protein spots derived particularly from hemocytes and 32 spots from hepatopancreas. These proteins may relate to the stress factors. However, the protein confirmation and link between the protein separations from 1D-SDS-PAGE and 2D-IEF to the quality of water and sediment are projected for future observation and validation.

Key words: Wild mud crabs, Scylla olivacea, Setiu wetland, proteomic approach

 

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