Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

46_03_08

E-mail Print PDF

Malays. Appl. Biol. (2017) 46(3): 59–65

BIOFILM FORMATION OF Salmonella enterica AND

THE CENTRAL ROLE OF RPOS SIGMA FACTOR IN

STRESS RESISTANCE

WAN ZAWIAH WAN ABDULLAH1,2*, BERNARD M. MACKEY1 and

KIMON-ANDREAS G. KARATZAS1

1Department of Food and Nutrition Sciences, University of Reading,

P.O. Box 226, Whiteknights, Reading RG6 6AP, UK

2School of Food Science and Technology, Universiti Malaysia Terengganu,

 

21030, Kuala Nerus, Terengganu, Malaysia

*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Accepted 23 August 2017, Published online 4 October 2017

 

ABSTRACT

Non-typhoidal Salmonella is considered as the leading cause of foodborne illness and it has been associated with highprofile outbreaks in many groups of foods. This work examines the contribution of phenotypic properties related to survival (biofilm formation) and how these are linked with the genetic and functional variability of rpoS gene and RpoS status respectively in Salmonella enterica. The test strains were Salmonella serovars Anatum, Enteritidis (466), Enteritidis (496), Hadar, Heidelberg, Montevideo, Newport and Virchow and two Typhimurium strains previously characterised as either RpoSpositive or RpoS-negative. RpoS status was found to affect biofilm formation. The capability of Salmonella to resist stress and survive under unfavourable conditions can vary between strains. We confirmed that the two strains that were previously sensitive to various stresses harboured significant mutations in the rpoS gene. The rpoS sequencing not only confirmed a link between RpoS and biofilm formation, but it also revealed a link with differences in the utilisation of carbon sources. The RpoS-negative phenotype was linked with an increased growth under different carbon sources suggesting that a functional RpoS is a burden for growth which is in agreement with the SPANC hypothesis.

Key words: Salmonella enterica, biofilm, RpoS

 

Latest MABJ issue

Vol 46(3) October 2017

Table of content

Latest news!

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.