Malaysian Applied Biology Journal

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46_01_14

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Malays. Appl. Biol. (2017) 46(1): 93–100


CHARACTERISATION OF POLYHYDROXYALKANOATE

PRODUCTION BY MUTANT Azotobacter  vinelandii

MOK, PEI SHZE1,2, SUDESH, KUMAR2, LIEW, PAULINE WOAN YING1*,

JONG, BOR CHYAN1 and NAJIMUDIN, NAZALAN2

1Agrotechnology and Bioscience Division, Malaysian Nuclear Agency,
Bangi, 4300 Kajang, Selangor, Malaysia
2School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden,
Pulau Pinang, Malaysia

*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Accepted 27 January 2017, Published online 31 March 2017

 

ABSTRACT

The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZ-Azotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as Avin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of 3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium by increasing the sucrose concentration from 20 g/L to 25 g/L.

Key words: Polyhydroxyalkanoate, Azotobacter vinelandii, mutant, nitrogen, sucrose concentration

 

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