Malaysian Applied Biology Journal

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46_01_13

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Malays. Appl. Biol. (2017) 46(1): 83–92


DYNAMIC CHANGES OF BACTERIAL COMMUNITY DURING

THE COMPOSTING OF OIL PALM EMPTY FRUIT BUNCHES

(OPEFB) AS ANALYZED BY DENATURING GRADIENT GEL

ELECTROPHORESIS (DGGE) ANALYSIS

JONG BOR CHYAN1*, GOH CHEE MENG1, LIEW PAULINE WOAN YING1

and THONG KWAI LIN2

1Agrotechnology and Bioscience Division, Malaysian Nuclear Agency, Bangi,
43000 Kajang, Selangor D.E., Malaysia
2Institute for Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

*Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Accepted 30 January 2017, Published online 31 March 2017

 

ABSTRACT

In this study cultivation-independent approach was performed to characterize and understand the bacterial community dynamics during the oil palm empty fruit bunches (OPEFB) composting process. OPEFB was composted in a compost heap of 1m × 1m × 1m. During the process, bacterial community structure in specific composting time were characterized by denaturing gradient gel electrophoresis (DGGE) analysis based on the hypervariable V3 region of 16S rRNA gene. The DGGE banding patterns revealed significant change in the bacterial community structure throughout the composting process. A total of 38 bands were selected for sequencing analysis. Majority of the DGGE bands’ sequences were related to known counterparts with low percentage of identity indicating novel composting bacterial populations. These DNA sequences were related to culturable relatives of Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Fibrobacteres and Chloroflexi. In general, distinctive predomination by Firmicutes followed by succession by Proteobacteria were revealed during the OPEFB composting process. Information attained in this study may be useful for improving the efficiency of OPEFB composting by indigenous bacterial population. The diverse sequences from known bacteria indicated novelty in the composting bacterial populations and potentially their functions.

Key words: Oil palm empty fruit bunches (OPEFB), compost, bacterial communities, denaturing gradient gel electrophoresis (DGGE), 16S rDNA

 

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