Malaysian Applied Biology Journal

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49_05_05

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Malays. Appl. Biol. (2020) 49(5): 41–52

 

GENETIC DIVERSITY OF NATURALLY DISTRIBUTED

Rhododendron moulmainense Hook. f. POPULATIONS IN

LAM VIEN PLATEAU, VIETNAM REVEALED BY

ISSR AND SCoT MARKERS


LUU THE TRUNG1,2, LA ANH DUONG1, TRAN VAN TIEN3,

LE NGOC TRIEU3 and PHI HONG HAI1*


1Vietnamese Academy of Forest Science, Duc Thang, Tu Liem, Hanoi, Vietnam

2Forest Science Institute of central Highlands and South of central Viet Nam, 09 Hung Vuong, Dalat,

Lam Dong, Vietnam, Vietnamese Academy of Forest Science

3Faculty of Biology, Dalat University, 01 Phu Dong Thien Vuong, Dalat, Lam Dong, Vietnam

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Accepted 11 October 2020, Published online 31 December 2020

 

ABSTRACT

Genetic diversity and variation of three naturally distributed Rhododendron moulmainense populations in the Lam Vien Plateau of Vietnam were analyzed using ISSR and SCoT markers separately and data from the two types of markers were then combined to show the overall results. Based on combined data, the genetic diversity parameters of the Tuyen Lam population were percentage of polymorphic bands (PPB) = 28.79%; genetic heterozygosity (He) = 0.1041; Shannon index (I) = 0.1552. The corresponding parameters were PPB = 37.88%; He = 0.1434; I = 0.2131 in the Hon Nga population, and PPB = 46.21%; He = 0.1857; I = 0.2709 in the Bidoup population. At the species level in Lam Dong province, the corresponding parameters were PPB = 62.12%; He = 0.1747; I = 0.2756. The average of pairwise genetic similarity coefficients among investigated individuals of Tuyen Lam population was 0.973, of Hon Nga population was 0.911, and of Bidoup population was 0.895. The genetic differentiation and the number of migrants per generation among investigated populations were GSTS = 0.1735 and Nm = 2.3824 individuals, respectively. Genetic distance between Tuyen Lam and Hon Nga populations was 0.0399, between Tuyen Lam and Bidoup populations were 0.0621, and Bidoup and Hon Nga populations were 0.0670. Data generated by both marker types and the combined revealed similar trends of genetic diversity levels among the studied populations. The SCoT data showed higher values of genetic diversity and population gene differentiation than did the ISSR data, but the latter showed higher gene differentiation among sampled individuals in the study populations. Comparing the values of Polymorphism Information Content (PIC) for discriminatory ability; Effective Multiplex Ratio (EMR) for several revealed loci and/or efficiency of the primer-marker system; Marker Index (MI) for the efficiency of used method; and Resolving Power (Rp) between used techniques indicated that discriminatory ability, efficiency, and ability of the ISSR primer combinations were higher but using SCoT marker system was more efficient than ISSR marker system for evaluating genetic diversity.


Key words: Rhododendron moulmainense, ISSR, SCoT genetic diversity, Vietnam

 

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Vol 49(5) December 2020

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