Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size

49_03_11

E-mail Print PDF

Malays. Appl. Biol. (2020) 49(3): 87–93

 

IDENTIFICATION OF METABOLITE PROFILE IN HALAL

AND NON-HALAL BROILER CHICKENS USING

FOURIER-TRANSFORM INFRARED SPECTROSCOPY (FTIR)

AND ULTRA HIGH PERFORMANCE LIQUID

CHROMATOGRAPHY- TIME OF FLIGHT- MASS

SPECTROMETRY (UHPLC-TOF-MS)

 

NURFATIN SYAHIRAH MOHAMED ALI1, ‘ATIQAH RUQAYYAH ZABIDI1,

MOHD NAZMI ABD MANAP1, SHIKH MOHD SHAHRUL NIZAN SHIKH ZAHARI2

and NAZARIYAH YAHAYA1,3*


1Programme of Food Biotechnology, Faculty of Science and Technology, Universiti Sains Islam

Malaysia, 71800, Nilai, Negeri Sembilan, Malaysia

2Programme of Industrial Chemical Technology, Faculty of Science and Technology,

Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan, Malaysia

3International Fatwa and Halal (iFFAH) ,Universiti Sains Islam Malaysia,

71800, Nilai, Negeri Sembilan, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 15 September 2020, Published online 25 October 2020


ABSTRACT

In Malaysia halal meat is fully defined by Jabatan Kemajuan Islam Malaysia (JAKIM) based on the killing method. Halal meat is usually associated with quality as Muslim sought meat from animal killed using Islamic method. In order to ensure the food are meeting the Halal and thoyyiban aspects, the procedure must be monitored along the supply chains beginning from farm to fork. However, there are lack of studies on effect of slaughtering methods on chickens’ metabolite profile. Therefore, metabolomics approach by Fourier-Transform Infrared Spectroscopy (FTIR) And Ultra High Performance Liquid Chromatography- Time of Flight- Mass Spectrometry (UHPLC-TOF-MS) are used in this study to understand the metabolite profile of chickens when subjected to different slaughtering process. The broiler chickens were subjected to Halal (Islamic tradition) and non-Halal slaughtering method (neck poking) where pectoral major muscle tissues from the slaughtered meat were selected for FTIR and UHPLC-TOF-MS analysis. Results from FTIR analysis showed Halal and non-Halal chicken displayed different spectra regardless time of extraction, which was 0 and 24 hours. Spectra obtained from UHPLC-TOFMS were further analyzed for statistical analysis, which are Principal Component Analysis (PCA) and Partial Least-Squares Discriminant Analysis (PLS-DA) using MetaboAnalyst 4.0. PLS-DA model showed higher intensity of histidine and inosine was recorded in non-Halal chicken while Halal chicken has higher concentration of hypoxanthine. Result from this study indicates that method of slaughter affects the metabolite profile of chicken.


Key words: Broiler chicken, halal, non-halal, slaughtering, metabolite profile, FTIR, UHPLC-TOF-MS, PCA.PLS-DA

 

Latest MABJ Issue

Vol 49(3) October 2020

coverphoto

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Elsevier, Mycite, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.