Malaysian Applied Biology Journal

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49_03_10

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Malays. Appl. Biol. (2020) 49(3): 81–85

 

MICROSATELLITE MARKERS FOR THE MOLECULAR

CHARACTERISATION OF POTENTIALLY COMMERCIAL

MANGO (Mangifera indica) PROGENIES


SHAHRIL AB RAZAK1*, MUHAMMAD AFIQ TAJOL ARIFFIN2,

SHARIFAH MUNIRAH SYD MOHAMAD1, NOR HELWA EZZAH NOR AZMAN1,

MOHD AZHAR HASSAN3 and JOHARI SARIP3


1Biotechnology and Nanotechnology Research Centre, MARDI Headquarters,

43400 Serdang, Selangor, Malaysia

2Horticulture Research Centre, MARDI Sintok, 06050, Bukit Kayu Hitam, Kedah, Malaysia

3Horticulture Research Centre, MARDI Headquarters, 43400 Serdang, Selangor, Malaysia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it or This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 2 September 2020, Published online 25 October 2020


ABSTRACT

Mango, or Mangifera indica as it is scientifically known, is a fruit crop that has significant importance to the global economy. This study characterised 10 commercially potential mango progenies using a total of 10 SSR markers. The analysis of the 10 SSR markers revealed the presence of 34 alleles. Each locus had 2–6 alleles equal to an average of 3.40 alleles per locus. The data also showed 0.115 (MiIIHR21) to 0.692 (MiIIHR28) polymorphic information content (PIC), equal to an average of 0.431. Each locus had heterozygosity and gene diversity values ranging from 0.044 (M1) to 0.957 (MiIIHR29) and from 0.122 (MiIIHR21) to 0.730 (MiIIHR29), respectively, averaging 0.480 and 0.476 each. The range of the pairwise genetic distance was 0.000 (ND014?ND019) to 0.611 (ND020?SB003). The dendrogram analysis showed that each mango progeny was successfully differentiated using the 10 SSR markers except for ND014 and ND019. The allele combination using these 10 SSR markers could be used to develop a DNA profile for fingerprinting purposes for each progeny except for ND014 and ND019, which can only be differentiated with the addition of an extra marker. The developed DNA fingerprinting profile could be used for cultivar identification and authentication. Additionally, breeders could also use the developed DNA profiles for plant variety protection (PVP) purposes.


Key words: DNA fingerprinting, genetic relationship Mangifera indica, SSR marker

 

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