Malaysian Applied Biology Journal

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49_02_13

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Malays. Appl. Biol. (2020) 49(2): 81–92

 

ISOLATION AND CHARACTERIZATION OF PISANG TONGKAT

LANGIT (Musa troglodytarum L.) ACS1 AND ACO1 GENE

EXPRESSION DURING FRUIT RIPENING PROCESS


FENNY MARTHA DWIVANY1,2,3*, KRISTI LENCI PATTY1,4 and CINDY NOVIANTI1


1School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Indonesia

2Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung, Indonesia

3Bali International Research Center for Banana, Indonesia

4Department of Agrotechnology, Faculty of Engineering and Agriculture,

Universitas Nani Bili Nusantara, Sorong, West Papua, Indonesia

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 25 April 2020, Published online 6 July 2020


ABSTRACT

Pisang tongkat langit (Musa troglodytarum L.) is a banana cultivar originated from Eastern Indonesia. Previous studies showed that ACS1 and ACO1 are genes that play an important role in banana ethylene biosynthesis. However, characterization and analysis of M. troglodytarum ACS1 and ACO1 genes have not been reported. This study aims to isolate and characterize the ACS1 and ACO1 gene expression profile during fruit ripening process. Gene fragment isolation was performed using PCR based method. Results showed that the isolated genomic fragments of ACS1 was 400 bp and ACO1 was 500 bp. cDNA fragments of ACS1 was 200 bp and ACO1 was 300 bp. Analysis of genomic fragments using BLAST NCBI showed that ACS1 had a high homology with 1818 bp ACS1 gene of Musa acuminata AAA Group (GenBank: GQ396304.1), while ACO1 gene had the highest homology with 1593 bp ACO1 gene of M. acuminata AAA Group (GenBank: AJ223232.1). ACS1 genomic fragments of M. troglodytarum had one intron and two exons, while ACO1 had two introns and three exons. Homology analysis showed that gene fragment of ACS1 and ACO1 of M. troglodytarum had conserved region similar to M. acuminata.


Key words: Pisang tongkat langit, Musa troglodytarum, ACS1, ACO1, fruit ripening

 

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