Malaysian Applied Biology Journal

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49_02_03

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Malays. Appl. Biol. (2020) 49(2): 13–17

 

CLONING AND in silico STUDIES OF LIPASE GENE OF A

THERMOPHILIC BACTERIA ISOLATED FROM THE

HYDROTHERMAL VENT AREA OF KAWIO, NORTH SULAWESI


KHALILAN LAMBANGSARI1, MAELITA RAMDANI MOEIS1 and ELVI RESTIAWATY2,3*


1School of Life Science and Technology, Institute of Technology Bandung,

Ganesha 10 Bandung 40132

2Research Group of Chemical Engineering Design and Development,

Faculty of Industrial Technology, Institute of Technology Bandung,

Ganesha 10 Bandung 40132

3Biosciences and Biotechnology Research Center, Institute of Technology Bandung,

Ganesha 10 Bandung 40132

*E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Accepted 7 May 2020, Published online 6 July 2020


ABSTRACT

Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated from marine water was a member of family I.4 of ‘true lipase’ group.


Key words: Lipase, thermophlic bacteria, hydrothermal vent, Bacillus safensis

 

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