Malaysian Applied Biology Journal

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28-1&2-20

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Malays. Appl. Biol. (1999) 28(1&2): 113-118

CORRELATION OF CA2+ ION AND ACROSOME REACTION WITH PRESENTATION OF Ml FUSION PROTEIN IN HAMSTER SPERMATOZOA

MAHANEM MAT NOOR1*, M.G. JUMAIDAH1 and H.D.M.MOORE2

1Department of Zoology, Faculty of Life Sciences, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
2Department of Molecular Biology and Biotechnology and Obsterics and Gynaecology, University of Sheffield, Sheffield S10 2UH, UK

ABSTRACT

Advances in many areas of reproductive technology have been rapid and, in many respects, have outstripped oui knowledge of the fundamental processes of human and animal sperm-egg interaction at fertilization. This is particularly true of human fertilization where the availability of eggs for research purposes is severely restricted. As consequence of this, most of the significant advances in our understanding of mammalian fertilization have resulted from studies or animals and particularly rodents. Previously antigen which involved in hamster sperm-egg fusion have been identified. The sperm antigen (designated Ml) localized specifically to the equatorial segment (ES) of hamster spermatozoa. In the present study the experiment was designed to examine the importance of Ca2+ and acrosome reaction to the presentation of Ml fusion protein on (ES) of viable and non-viable sparmatozoa. Sperm samples were incubated in different concentration of Ca2+ (in BWW medium). By indirect immunofluorescent technique it was found that at 1.7mM and 2.0mM fluorescent staining was present brightly on ES. Only sperm at this two concentrations undergo true AR and these have been observed by Aniline-blue Eosin B staining technique. The results suggest that the presentation of Ml fusion protein on ES of hamster spermatozoa is Ca2+ and AR dependent.


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