Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size


E-mail Print PDF
Malays. Appl. Biol. (1999) 28(1&2): 107-111



School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia


The purpose of this study was to investigate the effect of styrylpyrone derivative (SPD), on proliferation of ovarian cancer cell line Caov-3. Besides that, the expression level of bcl-2 and box in treated and untreated cells was also being looked into. The normal kidney cells such as Vero and MDBK were used for comparative purposes. Experiments on cell proliferation had shown that SPD was better than tamoxifen in inhibiting the growth of ovarian cancer cells withoul disturbing the proliferation of normal cells. mRNA Caov-3 cells was extracted in order to examine the expression oi bcl-2 and box genes after treatment with 10'6 M, 10'7 M and 10'8 M of SPD by using RT-PCR method, and the PCR product was sequenced. PCR product for bax gene was found to be approximately 323 bp in size. The sequenced nucleotide had been compared with database and was found to have high homology with human bax gene (93%). However there was no band detected for bcl-2 gene. In conclusion, SPD may contribute its effect through maintaining the expression of bax while preventing the expression of bcl-2 in Caov-3 cells. This finding was thought to be important because bax and bcl-2 are two oncogenes that are involved in the regulation of apoptosis in cancer cells.

Key words: Bcl-2, Bax, apoptosis, Ovarian cancer


Azimahtol, H.L.P., Johnson, S. & Laily, D. 1998. Non-steroid receptor mediated antiproliferative activity of styrylpyrone derivative in human breast cancer cell lines. Anticancer Res. 18: 1739-1744.

Bargou, R.C., Daniel, P.T., Mapara, M.Y., Bommert, K., Wagener, C., Kallinich, B., Royer, H.D. & Dorken, B. 1995. Expression of the bcl-2 gene family in normal and malignant breast tissue: low bax-a expression in tumor cells correlates with resistance towards apoptosis. Int. J. Cancer 60: 854-859.

Bartek, J., Bartkova, J. & Vojitesek, B. 1991. Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of malignancies. Oncogene 6: 1699-1703.

Ellis, R.E., Yuan, J. & Horvitz, H.R. 1991. Mechanisms and functions of cell death. Ann. Rev. Cell Biol. 7: 663-698.

Lin, L. & Hwang, P.L. 1991. Antiproliferative effects of oxygenated sterols: positive correlation with binding affinities for the antiestrogen-binding sites. Biochim .Biophy. Acta 1082: 177-184.

Marx, D., Binder, C., Meden, H., Lenthe, T., Ziemek, T., Hiddemann, T., Kuhn, W. & Schauer, A. 1997. Differential expression of apoptosis associated gene bax and bcl-2 in

ovarian cancer. Anticancer Research 17: 2233-2240.

Miyashita, T., Krajewski, S., Krajewski, M.: Wang, H.G., Lin, H.K., Liebermann, D.A Hoffmann, B. & Reed, J.C. 1994. Tumoi suppressor p53 is a regulator of bcl-2 and ban gene expression in vitro and in vivo. Oncogene 9: 1799-1805.

Oltvai, Z.N., Milliman, C.L. & Korsmeyer, S.J. 1993. Bcl-2 heterodimerizes in vivo with a conserved homolog Bax, that accelerates programmed cell death. Cell 74: 609-619.

Reed, J., Tsujimoto, Y., Epstein, S., Cuddy, M., Slabiak, T., Nowell, P. & Croce, C. 1989. Regulating of bcl-2 gene expression in lymphoid cell lines containing t(14;18) or normal #18 chromosomes. Oncogene Res. 4: 271-282.

Strasser, A, David, H.C.S. & Vaux, D.L. 1997. The role of the bcl-2 / ced-9 gene family in cancer and general implications of defects in cell death control for tumourigenesis and resistance to chemotherapy. Biochi. et Biophy. Acta 1333: F151-178.

Tsujimoto, Y. & Croce, C.M. 1986. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. USA 83: 5214-5218.

Latest MABJ Issue

Vol 48(1) March 2019

Table of content

Latest news!

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.