Malaysian Applied Biology Journal

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28-1&2-19

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Malays. Appl. Biol. (1999) 28(1&2): 107-111

EFFECTS OF STYRYLPYRONE DERIVATIVE (SPD) ON EXPRESSION OF BCL-2 AND BAX GENES IN HUMAN OVARIAN CARCINOMA CELL LINE, CAOV-3

TEOH, P. L.* and AZIMAHTOL HAWARIAH, L. P.

School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia

ABSTRACT

The purpose of this study was to investigate the effect of styrylpyrone derivative (SPD), on proliferation of ovarian cancer cell line Caov-3. Besides that, the expression level of bcl-2 and box in treated and untreated cells was also being looked into. The normal kidney cells such as Vero and MDBK were used for comparative purposes. Experiments on cell proliferation had shown that SPD was better than tamoxifen in inhibiting the growth of ovarian cancer cells withoul disturbing the proliferation of normal cells. mRNA Caov-3 cells was extracted in order to examine the expression oi bcl-2 and box genes after treatment with 10'6 M, 10'7 M and 10'8 M of SPD by using RT-PCR method, and the PCR product was sequenced. PCR product for bax gene was found to be approximately 323 bp in size. The sequenced nucleotide had been compared with database and was found to have high homology with human bax gene (93%). However there was no band detected for bcl-2 gene. In conclusion, SPD may contribute its effect through maintaining the expression of bax while preventing the expression of bcl-2 in Caov-3 cells. This finding was thought to be important because bax and bcl-2 are two oncogenes that are involved in the regulation of apoptosis in cancer cells.


Key words: Bcl-2, Bax, apoptosis, Ovarian cancer

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