Malaysian Applied Biology Journal

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28-1&2-17

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Malays. Appl. Biol. (1999) 28(1&2): 99-104

ASSESSMENT OF DETOXIFYING ENZYMES IN CALLOSOBRUCHUS MACULATUS RESISTANCE AGAINST LEGUMES

JANARTHANAN, S*., VENUGOPAL K.J. and S. IGNACIMUTHU

Entomology Research Institute Loyola College, Chennai - 600 034, Tamil Nadu, India

ABSTRACT

Investigations have been carried out to evaluate the relationship between secondary anti-nutrient/anti-bruchid compounds present in some important pulses and three major insect detoxification enzymes namely, glutathione S-transferase, the cytochrome P-450 and carboxylesterases in the bruchid beetle, Callosobruchus maculatus, an important stored grain pest of pulses. Ten varieties of wild and ten varieties of cultivar pulses were allowed separately to be infested by the beetles for ten generations. The glutathione S-transferase, cytochrome P-450 and carboxylesterase activities from the larval mid-guts of such insects were compared and correlated to the levels of anti-bruchid compounds (trypsin inhibitor, chymotrypsin inhibitor and a-amylase inhibitor) present in the seeds. Our results show that activities of these detoxification systems were higher when the larvae were fed on the wild varieties that contained higher amounts of these anti-bruchid compounds.


Key words: Callosobruchus maculatus, glutathione S-transferase, cytochrome P450, carboxylesterase, pulses, resistance, anti-bruchid compounds.

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