Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size


E-mail Print PDF
Malays. Appl. Biol. (1999) 28(1&2): 99-104



Entomology Research Institute Loyola College, Chennai - 600 034, Tamil Nadu, India


Investigations have been carried out to evaluate the relationship between secondary anti-nutrient/anti-bruchid compounds present in some important pulses and three major insect detoxification enzymes namely, glutathione S-transferase, the cytochrome P-450 and carboxylesterases in the bruchid beetle, Callosobruchus maculatus, an important stored grain pest of pulses. Ten varieties of wild and ten varieties of cultivar pulses were allowed separately to be infested by the beetles for ten generations. The glutathione S-transferase, cytochrome P-450 and carboxylesterase activities from the larval mid-guts of such insects were compared and correlated to the levels of anti-bruchid compounds (trypsin inhibitor, chymotrypsin inhibitor and a-amylase inhibitor) present in the seeds. Our results show that activities of these detoxification systems were higher when the larvae were fed on the wild varieties that contained higher amounts of these anti-bruchid compounds.

Key words: Callosobruchus maculatus, glutathione S-transferase, cytochrome P450, carboxylesterase, pulses, resistance, anti-bruchid compounds.


Ahmed, S., Brattsten, Mullin, C. A. and Yu, S. J. 1986. Enzymes involved in the metabolism of plant allelochemicals. In: Molecular Aspects of Insect - Plant Associations. L. B. Brattsten and S. Ahmed (eds.). Plenum Press, New York. pp. 73-152.

Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utlilising the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.

Clarke, A. G. and Shamaan, N. 1984. Evidence that DDT-dehydrocholorinase from house fly is a glutarhione S-transferase. Pesticide Biochemistry and Physiology 22: 249-261. Devonshire, A. L. 1975. Studies of the carboxylesterases of Myzus persicae resistant and susceptible to organophosphorus insecticides. In: Proceedings of the 8th British Insecticide and Fungicide Conference, pp 67-73.

Dobie, P. 1980. Use of resistant varieties of cowpeas (Vigna unguiculata) to reduce losses due to post-harvest attack by Callosobruchus maculatus In: The ecology of Bruchids Attacking Legumes, V. Labeyrie (ed.). The Hague.

Gatehouse, A. M. and D. Boulter 1983. Assessment of the anti-metabolic effects of trypsin inhibitors from cow pea (Vigna unguiculata) and other legumes on development of the bruchid beetle Callosobruchus maculatus. Journal of Science and Food Agriculture 34: 345-350.

Gatehouse, A. M., Butler, K. J., Fenton, K. A. and Gatehouse, J. A. 1985. Presence and partial characterization of a major proteolytic enzymes in the larval gut of Callosobruchus maculatus. Entomologia Experimentalis et Applicata 39: 279-286.

Gatehouse, A. M., Minney, H., Dobie, P. and Hilder, V. A. 1990. Biochemical resistance to bruchid attack in legumes: investigation, and exploitation. In Bruchids and Legumes: Economics, Ecology and Coevolution. K. Fujil, A. M. Gatehouse, C. D. Johnson, R. Mitchel and T. Yoshida (eds). Kluwer Academic Publishers, Dordrecht, pp. 241-256.

Gatehouse, A. M., Gatehouse, J. A., Dobie, P., Kilminster, A. and Boulter, D. 1979. Biochemical basis of insect resistance in Vigna unguiculata. Journal of Science and Fooa Agriculture 30: 948-958.

Ignacimuthu, S., Janarthanan, S. and Balachandran, B. 1999. Chemical basis of resistance in pulses towards Callosobruchus maculatus (F.). Journal of Stored Product Research 00: 1-11.

Janzen, D. H., Ryan, C. A., Liener, I. E. and Pearce, G. 1986. Potentially defensive proteins in mature seeds of 59 speices of tropical leguminosae. Journal of Chemical Ecology 12: 1469-1480.

Lee, K. 1991. Glutathione S-transferase activities in phytophagous insects: induction and inhibition by plant phototoxins and phenols. Insect Biochemistry 21: 353-361.

Leszczynski, B., Matok, H. and Dixon, A. F. G. 1992. Resistance of cereals to aphids :The interaction between hydroxamic acids and UDP-glucose transferases in the aphid Sitobion avenage (Homoptera: ApMdidae). Journal of Chemical Ecology 18: 1189-1200.

Lindroth, R. L. 1989a. Chemical ecology of the luna moth: Effects of host plant on detoxification enzyme activity. Journal of Chemical Ecology 15: 2019-2029.

Lindroth, R. L. 1989b. Host plant alteration of detoxification activity in Papilio glaucus glaucus. Entomologia Experimentalis et Applicata 50: 29-35.

Lindroth, R. L., Jung, S. M. and Feuker, A. M. 1993. Detoxification activity in the gypsy moth: Effects of host CO2 and NO3 availability. Journal of Chemical Ecology 19: 357-367.

Messina, F. J. and Renwick, J. A. A. 1985. Resistance of Callosobruchus maculatus (Coleoptera: Bruchidae) in selected cowpea lines. Environmental Entomology 14: 868-872.

Murdock, L. L., Brookhart, G., Dunn, P. E., Foard, D. E., Kelly, S., Kitch, L., Shade, R. E., Shukle, R. H. and Wolfson, J. L. 1987. Cysteine digestive proteinases in Coleoptera. Comparative Biochemistry and Physiology 87B: 783-787.

Omura, R. and Sato, R. 1964. The carbon monoxide binding pigment of liver microsomes. II solubilization, purification and properties. Journal of Biological Chemistry 239: 2379-2385.

Owusu, E. 0., Horiike, M. and Hirano, C. 1996. Inhibition of insecticides of partially purified carboxylesterase from Aphis gossypii (Homoptera : Aphididae). Journal of Economic Entomology 89: 307-310.

Latest MABJ Issue

Vol 48(3) June 2019

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Myjurnal, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.