Malaysian Applied Biology Journal

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Malays. Appl. Biol. (1999) 28(1&2): 85-92



Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia


The polymerase chain reaction (PCR) technique was employed to amplify a region of 778 bp of the glycoprotein B ,(gB] gene of pseudorabies virus (PrV). The specificity of the primers was confirmed by testing them on various PrV strains, isolates and a thymidine kinase defective PrV. The technique was applied on infected mouse tissues either primarily infected or re-infected with PrV. DNA samples from all PrV isolates were found to be positive. Mouse tissues were also tested and found to be positive on day 3 and 5 post-inoculation. When comparing PCR to virus isolation method, some samples were found to be positive for virus isolation and PCR. However, some of the samples were negative for virus but were positive for PCR technique. This indicated that PCR technique employed was more sensitive and specific than the later.

>Ali, A.S., Mohd-Azmi, M.L. and Aini, I. 1998. Immune responses to a non-pathogenic pseudorabies virus (mAlp) in mice. J. Vet. Malaysia 10(2): 55-62.


Ali, A.S., Mohd-Azmi, M.L. and Aini, I. 1998. Immune responses to a non-pathogenic pseudorabies virus (mAlp) in mice. J. Vet. Malaysia 10(2): 55-62.

Babic, N., Metteneliter, T.C., Ugolini, G., Flamand, A. and Coulon, P., 1994. Propagation of pseudorabies virus in nervous system of the mouse after intranasal inoculation. Virology, 204: 616-625.

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Robbins, A.K., Dorney, D.J., Wathen, M.W. Whealy, M.E., Gold, C., Watson, R.J., Holland, L.E., Weed, S.D., Levine, M., Glorioso, J.C. and Enquist, L.W. 1987. The pseudorabies virus gll gene is closely related to the gB glycoprotein gene of herpes simplex virus. J. Virol. 61: 2691-2701.

Rock, D.L., Hagemoser, W.a., Osorio, F.A. and McAllister, H.A. 1988. Transcription from the pseudorabies virus genome during latent infection. Arch. Virol. 98: 99-106.

Rziha, H.J., Mettenleiter, T.C., Ohlinger, V. and Wittman,G. 1986. Herpesvirus (pseudorabies virus) latency in swine: Occurrence and physical state of viral DNA in neural tissues. Virology, 155: 600-613.

Saiki, R.K., Gelfand, H.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Ehrlich, H.A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239: 487-491.

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Wittmann, G. 1989. Aujeszk's disease (pseudorabies) in ruminants. In Herpesvirus Diseases of cattle, Horeses and pigs (Edited by Wittmann, G.). pp. 163-175. Kluwer, Baston.

Wittmann, G. and Rziha, H.J. 1989. Aujeszky's disease (pseudorabies) in pigs. In Herpesvirus Diseases of Cattle, Horses and Pigs (Edited by Wittmann, G.), pp. 230-325. Kluwer, Baston.

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