Malaysian Applied Biology Journal

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28-1&2-15

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Malays. Appl. Biol. (1999) 28(1&2): 85-92

POLYMERASE CHAIN REACTION FOR DETECTION OF PSEUDORABIES VIRUS DNA IN INFECTED MOUSE TISSUES

M.L. MOHD-AZMI, A.S. ALI, N. MUSA and I. AIM

Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

ABSTRACT

The polymerase chain reaction (PCR) technique was employed to amplify a region of 778 bp of the glycoprotein B ,(gB] gene of pseudorabies virus (PrV). The specificity of the primers was confirmed by testing them on various PrV strains, isolates and a thymidine kinase defective PrV. The technique was applied on infected mouse tissues either primarily infected or re-infected with PrV. DNA samples from all PrV isolates were found to be positive. Mouse tissues were also tested and found to be positive on day 3 and 5 post-inoculation. When comparing PCR to virus isolation method, some samples were found to be positive for virus isolation and PCR. However, some of the samples were negative for virus but were positive for PCR technique. This indicated that PCR technique employed was more sensitive and specific than the later.


>Ali, A.S., Mohd-Azmi, M.L. and Aini, I. 1998. Immune responses to a non-pathogenic pseudorabies virus (mAlp) in mice. J. Vet. Malaysia 10(2): 55-62.

REFERRENCES

Ali, A.S., Mohd-Azmi, M.L. and Aini, I. 1998. Immune responses to a non-pathogenic pseudorabies virus (mAlp) in mice. J. Vet. Malaysia 10(2): 55-62.

Babic, N., Metteneliter, T.C., Ugolini, G., Flamand, A. and Coulon, P., 1994. Propagation of pseudorabies virus in nervous system of the mouse after intranasal inoculation. Virology, 204: 616-625.

Ben-Porat, T., and Kaplan, A.S., 1985. Molecular Biology of Pseudorabies virus. In: The herpesviruses (Edited by Roizman, B.), vol. Ill, pp. 105-173. Plenum Press, New York.

Brown, T.M., Osorio, F.A. and Rock, D.L. 1990. Detection of latent pseudorabies virus in swine using in situ hybridization. Vet. Microbiol. 24: 273-280.

Conraths, F.J., Pauli, G. and Ludwig, H., 1988. Monoclonal antibodies directed against a 130k glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein B of herpes simplex virus. Virus Res., 10:53-64.

Crandell, R.A., 1982. Pseudorabies (Aujeszky's disease). Vet. Clin. North Am: Large Animal Practice, 4: 321-331.

Eich, K.O., 1983. Die Aujeszkysche Krankheit des Schweines. Tieraertzl. Praxis, 11: 323-330.

Field, H.J and Hill, T.J. 1974. The pathogenesis of pseudorabies virus in mice following peripheral inoculation. J.Gen.Virol. 23: 145-157.

Hermann, G. and Frischauf, A.-M, 1987. Isolation of genomic DNA. In: S. Ber>xi and A. Kimmel (Editors), Methods in Enzymology, Volume 152. Guide to molecular cloning techniques. Academic Press, Orlando, pp. 180-183.

Hoblet, K.H., Miller, G.Y. and Bartter, N.G. 1987. Economic assessment of pseudorabies enzootic, breeding herd removal/ repopulation and downtime in a commercial swine herd. J. Am. Vet. Med. Assoc. 190: 405-409.

Jestin, A. 1990. Rapid detection of pseudorabies genomic sequences in biological samples from infected pigs using PCR DNA amplification. Vet. Microbiol. 23: 317-328.

Johnson, M.E., Thawley, , D.G. , Solozano, R.F. and Wright, J.C., 1983. Evaluation of the immunodiffusion test for the detection of antibody to pseudorabies virus. Am. J. Vet. Res., 44: 28-30.

Jons, A., Granzow, H., Kuchling, R., Mettenleiter, T.C. 1996. The UL49.5 gene of paeudorabies virus codes for an O-glycosylated structural protein of the viral envelope. /. Virol. 70: 1237-1241.

Maes, R.K., Beisel, C.E., Spatz, S.J. and Thacker, B.J. 1990. Polymerase chain reaction amplification of pseudorabies virus DNA from acutely and latently infected cells. Vet. Microbiol. 24: 281-295.

Maes, R.K., Spatz, S.J. and Thacker, B.J. 1988. Nonradioactive probes to detect acute and latent pseudorabies virus (Aujeszky's disease) infections. Proc. 10th IPVS Congress, Rio de Janeiro, Brazil, p. 169.

Mathews, R.E.F. 1982. Classification and nomenclature of viruses. Intervirology 17: 1-200.

McFarlane, R.G., Thawley, D.G. and Solorzano, R.F. 1986. Detection of latent pseudorabies virus in porcine tissues using a DNA hybridization dot-blot assay. Am. J. Vet. Res., 47: 2329-2336.

Mettenleiter, T.C. 1994. Pseudorabies (Aujeszky's) virus: state of the art. Acta Vet Hung 42: 153-177.

Nash, A.A., Field, H.J. and Quartery-Papation, R. 1980. Cell Mediated Immunity in Herpes Simplex Virus-Infected mice; induction, characterization and antiviral effects of delayed-type hypersensitivity. /. Gen. Virol. 48: 351-357.

Onno, M., Jestin, A. and Nicolas, J.C. 1988. Rapid diagnosis of Aujeszk's disease in fattened pigs by direct virus immunoperoxidase labelling in nasal cells. Vet. Med. Rev., 59: 152-156.

Pensaert, M.B. and Kluge, J.P. 1989. Pseudorabies virus in: Virus infections of Porcines. Edited by Pensaert, M.B. Elsevier, Sc. Publishers, Oxford. Pp.39.

Robbins, A.K., Dorney, D.J., Wathen, M.W. Whealy, M.E., Gold, C., Watson, R.J., Holland, L.E., Weed, S.D., Levine, M., Glorioso, J.C. and Enquist, L.W. 1987. The pseudorabies virus gll gene is closely related to the gB glycoprotein gene of herpes simplex virus. J. Virol. 61: 2691-2701.

Rock, D.L., Hagemoser, W.a., Osorio, F.A. and McAllister, H.A. 1988. Transcription from the pseudorabies virus genome during latent infection. Arch. Virol. 98: 99-106.

Rziha, H.J., Mettenleiter, T.C., Ohlinger, V. and Wittman,G. 1986. Herpesvirus (pseudorabies virus) latency in swine: Occurrence and physical state of viral DNA in neural tissues. Virology, 155: 600-613.

Saiki, R.K., Gelfand, H.H., Stoffel, S., Scharf, S.J., Higuchi, R., Horn, G.T., Mullis, K.B. and Ehrlich, H.A. 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239: 487-491.

Spear, P.O., 1993., Entry of alphaherpesviruses into cells. Semin. Virol. 4: 167-180.

Wittmann, G. 1989. Aujeszk's disease (pseudorabies) in ruminants. In Herpesvirus Diseases of cattle, Horeses and pigs (Edited by Wittmann, G.). pp. 163-175. Kluwer, Baston.

Wittmann, G. and Rziha, H.J. 1989. Aujeszky's disease (pseudorabies) in pigs. In Herpesvirus Diseases of Cattle, Horses and Pigs (Edited by Wittmann, G.), pp. 230-325. Kluwer, Baston.
 

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