Malaysian Applied Biology Journal

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28-1&2-14

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Malays. Appl. Biol. (1999) 28(1&2): 79-84

MENDELIAN INHERITANCE OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS IN THE RIVER CATFISH, MYSTUS NEMURUS

CHONG, L.K.1, TAN, S.G.1*, SIRAJ, S.S.1, CHRISTIANUS, A.1 and YUSSOF, K.2

1 Department of Biology 2 Department of Biochemistry and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.

ABSTRACT

The segregation and inheritance of random amplified polymorphic DNA (RAPD) markers were tested in 2 families of the river catfish Mystus nemurus. Five primers for family A and five for family B generated a total of 27 polymorphic markers that are suitable for segregation studies. Of these 27 markers, 19 (70%) segregated in Mendelian fashion and their Chi-square analyses showed agreement with the expected ratio, at the 5% significance level. Two markers were significantly different (P<0.05) and thus not considered to be genetic markers. Five markers showed unusual segregation where recessive phenotypes were observed among the F] progeny when none was expected. Additionally, one non-parental band that was amplified only in the offspring was noted. In conclusion, not all the scorable RAPD markers showed Mendelian inheritance. Markers that are genetically controlled and their primers could then be used for subsequeni analysis of the genetic structure of M. nemurus populations.


Key words: RAPD markers, Mendelian inheritance, Mystus nemurus

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