Malaysian Applied Biology Journal

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Malays. Appl. Biol. (1999) 28(1&2): 79-84



1 Department of Biology 2 Department of Biochemistry and Microbiology, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia.


The segregation and inheritance of random amplified polymorphic DNA (RAPD) markers were tested in 2 families of the river catfish Mystus nemurus. Five primers for family A and five for family B generated a total of 27 polymorphic markers that are suitable for segregation studies. Of these 27 markers, 19 (70%) segregated in Mendelian fashion and their Chi-square analyses showed agreement with the expected ratio, at the 5% significance level. Two markers were significantly different (P<0.05) and thus not considered to be genetic markers. Five markers showed unusual segregation where recessive phenotypes were observed among the F] progeny when none was expected. Additionally, one non-parental band that was amplified only in the offspring was noted. In conclusion, not all the scorable RAPD markers showed Mendelian inheritance. Markers that are genetically controlled and their primers could then be used for subsequeni analysis of the genetic structure of M. nemurus populations.

Key words: RAPD markers, Mendelian inheritance, Mystus nemurus


Bardakci, K. and Skibinski, D.O.F. (1994). Application of the RAPD technique in tilapia fish: species and subspecies identificaion. Heredity. 73: 117-123.

Foo, C.L., Dinesh, K.R., Lim, T.M., Chan, W.K. and Phang, V.P.E. (1995). Inheritance oi RAPD markers in the Guppy fish, Poecilia reticulata. Zoological Science, 12: 535-541.

Heun, M. and Helentjaris, T. (1993). Inheritance of RAPDs in f! hybrids of corn. Theoretical and Applied Genetics, 85: 961-968.

Hunt, G. J. and Page, R. E. Jr. (1995). Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics, 139: 1371-1382.

Hunt G.J. and Page R.E. Jr (1992) Patterns oi inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee. Theoretical and Applied Genetics, 85, 15-20.

Jansen, R. and Ledley, F.D. (1990). Disruption oi ohase during PCR amplification and cloning heterozygous target sequences. Nucleic Acids Research, 18: 5153-5156.

Liu, Z., Li, P., Argue, B.J. and Dunham, R.A. (1998). Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (/. Furcatus), and their Fj, F2 and backcross hybrids. Animal Genetics, 29: 58-62.

Martin G. B., Williams J. G. K. and Tanksley S.D. (1991). Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near isogenic lines. Proceedings of the National Academiy of Sciences, USA, 88: 2336-2340.

Novy, R. G. and Vorsa, N. (1996). Evidence of RAPD heteroduplex formation in cranberry: implications for pedigree and genetic-relatedness studies and a source of co-dominant RAPD markers. Theoretical and Applied Genetics, 92: 840-849.

Obara-Okeyo, P. and Kako, S. (1998) Genetic diversity and identification of Cymbidium cultivars as measured by random amplified polymorphic DNA (RAPD) markers. Euphytica, 99: 95-101.

Paran, I., Keseli, R. and Michelmore, R. (1991). Identification of restriction fragment length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenoc lines. Genome 34:1021-1027.

Promboon, A., Shimada, T., Fujiwara, H., and Kobayashi, M. (1995). Linkage map of random amplified polymorphic DNAs (RAPDs) in the silkworm, Bombyx mori. enet. Res.Camb, 66:1-7

Rafalski, J. A., Tingey, S. V. and Williams, J. G. K. (1991). RAPD markers - a new technology for genetic mapping and plant breeding. Agriculture Biotechnology News Info, 3: 645-648.

Reiter, R.S., William, J.G.K., Feldmann, K.A., Rafalski, J. A., Tingey, S. V., and Scolnik, P. A. (1992). Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymophic DNAs. Proceedings of the National Academy of Sciences, USA, 89: 1477-1481

Ronning, C. M. and Schnell, R. J. (1995) Inheritance of random amplified polymorphic DNA (RAPD) markers in Theobroma cacao. Journal of the American Society for Horticutural Science, 120: 681-686.

Schnell, R. J., Ronning, C. M., and Knight, R. J. Jr. (1995) Identification of cultivars and

validation of genetic relationships in Mangifera indica L. using RAPD markers. Theoretical and Applied Genetics, 90: 269-274.

Scott, M. P., Haymes, K. M. and Eilliams S. M. (1992). Parentage analysis using RAPD PCR. Nucleic Adds Research, 20: 5493.

Stott W., Ihssen P.E. and White B.N. (1997). Inheritance of RAPD molecular markers in lake trout. Salvelinus nameycush. Molecular Ecology. 6: 609-613

Strongaman, D. B. and Mackay, M. (1993). Discrimination between Hirsutella longicolla var. longicolla and Hirsutella colla var. cornuta using random amplified polymorphic DNA fingerprinting. Mycologia, 85: 65-70.

Sultamann, H., Mayer, W. E., Figueroa, F., Tichy, H., and Jan Klein, J. (1995). Phylogenetic analysis of cichlid fishes using nuclear DNA markers. Molecular Biology of Evolution, 12(6): 1033-1047.

Taggart, J. B., Hynes, R. A., Prodohl, P. A., Ferguson, A. (1992). A simplified protocol for routine total DNA isolation from salmonid fishes. Journal of Fish Biology, 40 : 964-965.

Tinker, N.A., Fortin, M.G. and Mather, D.E. (1993). Random amplified polymorphic DNA and pedigree relationships in spring barley. Theoretical and Applied Genetics, 85: 976-984

Welsh, J., Honeycutt, R. J., McClelland, M. and Sobral, B. W. S. (1991). Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theoretical and Applied Genetics, 82: 473-476.

Welsh, J. and M. McClelland (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research, 18: 7213-7218.

Williams, J.G.K., Kubelik, A.R., Livak K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535.

Williams, D.J., Kazianis, S. and Walter, R. B. (1998). Use of random amplified polymorphic DNA (RAPD) for identification of Laremouth Bass subspecies and their intergrades. Transactions of the American Fisheries Society, 127: 825-832.

Yang, X. and Quiros, C. (1993). Identification and classification of celery cultivars with RAPD markers. Theoretical and Applied Genetics, 86: 205-212.

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