Malaysian Applied Biology Journal

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28-1&2-12

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Malays. Appl. Biol. (1999) 28(1&2): 69-71

THE EFFECTS OF EXTRACELLULAR CALCIUM ON ACETYLCHOLINESTERASE ACTIVITY IN HARUAN, CHANNA STRIATUS AND COCKLES, ANADARA GRANOSA

ABDUL MANAN MAT JAIS, MUHAMMAD ZIN MOHAMED, HASIAH ABDUL HAMID and ABDUL SALAM ABDULLAH

Department of Biomedical Sciences, Faculty of Medical and Health Sciences, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor DE, Malaysia

ABSTRACT

This study was conducted as pan of a research project to establish acetylcholinesterase as a biomarker; and to understand the role of extracellular calcium in the mechanism of interaction of acetylcholinesterase in aquatic organisms. This is based on the hypothesis that the acetylcholinesterase in aquatic animals is different from that in terrestrial counterparts. Hainan, Channa striatus, or also known as snakehead, is a carnivorous and air breather freshwater fish. It is abundantly found in shallow slow running water in Malaysia. Haruan is a commercial food fish and is consumed by many to induce wound healing. All of the haruan used in this experiment were obtained from a farmer in Pontian, Johor, with body size of 20.65 + 6.00 cm in length and weighing between 72.20 + 12.55 g. Meanwhile, random marketable size cockles, Anadara granosa, a popular seafood, were bought from the local wet market. Acetylcholinesterase activity in both haruan brain tissue and cockle abductor muscles were measured according to the Ellman, et al., 1961 protocol using a Shimadzu UV Spectrophotometer. Subsequently, protein in both haruan brain tissue and cockle abductor muscles were determined based on the Lowry, et al., 1951 method. The brain and abductor muscle samples were homogenised separately in a sucrose-EDTA solution for the assay and bovine serum used as a standard. The highest acetylcholinesterase activity in haruan brain tissue was 1.961 ± 0.801 pmol/min/mg protein and the lowest was - 0.470 + 0.143 pmol/min/ mg protein. However, the acetylcholinesterase activity in the abductor muscles of cockles was slightly lower in the range of 1.731 + 0.151 pmol/min/mg protein the highest and - 0.380 ± 0.025 pmol/min/mg protein at the lowest. The brain and abductor muscle preparation were exposed to a series of extracellular calcium of 0.00 (control); 0.05; 0.25; 0.50; 0.75 and 1.00 ppm for 0; 10; 20 and 30 minutes during the assay to determine the influence of calcium on the acetylcholinesterase activity. The results indicate that extracellular calcium does not alter acetylcholinesterase activity in both the brain tissue of C. striatus and the abductor muscles of A. granosa.


Key words: Acetylcholinesterase activity, aquatic organisms; haruan, Channa striatus; cockles, Anadara granosa

REFERRENCES

Ellman, G.L.; K.D.Courtney; I.R.Andres and R.M.Featherstone, 1961. A New Rapid Calorimetric Determination of Acetyl­cholinesterase Activity. Biochem. Pharmacol., vol 7, pp 88 - 95.

Fulton. M.H and J.E.Chambers, 1985. Inhibition of Neurotoxic Esterase and Acetyl­cholinesterase by Organophosphorous Compounds in Selected Ecthothermic Vertebrates. Pest. Biochem. Physiol.,vo\23, pp 282 - 288.

Kanno, C.; J.K.Cho and Y.Tekeda, 1992. Divalent Cation and Electrical Properties of Cortisol Cell. J. Lowry, O.H.; N.J.Rosebrough; A.L.Farr and R.J.Randel, 195 1. Measurement of Protein With the Folin Phenol Reagent. J. Biol. Chem., vol 193, pp 265 - 275.

Mat Jais, A.M.; M.Muhamad; S.W.Ang and J.H.Arshad, 1995. In vitro Effects of Endosulfan on the Activity of Acetyl­cholinesterase in Brain Tissue of Haruan, Channa striatus. Proc. 5"' SET AC Europe Congress 1995.

Reddy, P.M.; G.H.Philip and M.D.Bashamohiden, 1992. Regulation of AChE System of Freshwater Fish, Cyprinus carpio, Under Fenvelerate Toxicity. Bull. Environ. Contam. Toxicol., vol 48, pp 18-22.

Wasserman, R.H., 1977. Calcium Binding Protein and Calcium Function, North Holland.
 

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