Malaysian Applied Biology Journal

  • Increase font size
  • Default font size
  • Decrease font size


E-mail Print PDF
Malays. Appl. Biol. (1999) 28(1 &2): 49-57



1 Department of Biotechnology 2 Department of Food Science, Faculty of Food Science and Biotechnology, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia


Two hundred ten strains of Escherichia coli isolated from litters of broiler (105 strains) and village (105 strains) chicken were examined for antibiotic resistance, plasmid profiles and random amplification of polymorphic DNA analysis. All were found to exhibit multiple resistance towards the antibiotics tested, with multiple antibiotic resistance index of 0.44 to 0.69 and 0.31 to 0.75 for the strains from broiler and village chicken, respectively, indicating that all strains originated from high risk sources. The E. coli strains from broiler and village chicken were grouped into 86 and 80 distinct antibiotypes. Ninety nine and 71 strains were found to harbour plasmids ranging in size from 1 to 80 kb and 1.3 to 64 kb among the broiler and village chicken isolates respectively. Based on their plasmid profiles, the E. coli strains isolated from broiler and village chicken were grouped into 60 and 27 plasmid patterns respectively. We used two single short primers (Genl5009 and GenlSOlO) with arbitrary nucleotide sequences in polymerase chain reaction to amplify genomic DNA. The profiles observed after electrophoretic separation for the two primers when combined together were able to distinguish the E. coli strains from broiler and village chicken into 96 and 92 RAPD patterns, respectively. These results suggest that RAPD-PCR assay and antibiotyping are more discriminating than plasmid profiling, and could be valuable tools for epidemiological studies.

Key words: Escherichia coli, RAPD, plasmid, antibiotic resistance


Anon. 1993. National Committee for Clinical Laboartory Standards. Performance standards for antimicrobial disc susceptibility tests: tentative standards, vol. 13, No. 24. NCCLS document M2-A5. National Committee for Clinical Laboratory Standards, Villanove, Pa.

Baya, A. M., Brayton, P. R., Brown, V. L., Grimes, D. J., Russek-Cohen, E. and Colwell, R. R. 1986. Coincident plasmids and antimicrobial resistance in marine bacteria isolated from polluted and unpolluted Atlantic Ocean samples. Applied and Environmental Microbiology 51: 1285-1291.

Beery, J. T., Doyle, M. P. and Schoeni, J. L. 1985. Colonization of chicken cecae by Escherichia coli asssociated with hemorrhagic colitis. Applied and Environmental Microbiology 49: 310-315.

Clark, A. G. and Lanigan, C. M. S. 1993. Prospects for estimating nucleotide divergence with RAPDs. Molecular Biology and Evolution 10: 1096-1111.

Geldreich, E. E. 1966. Sanitary significance of fecal coliforms in the environment. Water Pollution Control Research Series, publication WP-20-3. Federal Water Pollution Control Administration, U. S. Department of the Interior, Cincinnati, Ohio.

Krumperman, P. H. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Applied and Environmental Microbiology 46: 165-170.

Levine, M. M. 1987. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. Journal of Infectious Diseases 155: 377-389.

Macrina, F. L., Kopecko, D. J., Jones, K. R. and McCowen, S. M. 1978. A multiple-plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1:417-420.

Maslow, J. N., Brecher, S., Gunn, J., Durbin, A., Barlow, M. A. and Arbeit, R. D. 1995. Variation and persistence of methicillin-resistant Staphylococcus aureus strains among individual patients over extended periods ol time. European Journal of Clinical Micro­biology and Infectious Diseases 14: 282-290.

Sambrook, J., Fritsch, E. F. and Maniatis, M. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

Son, R., Ansary, A., Rusul, G. and Karim, M. I. A. 1996a. Isolation of verotoxin-producing Escherichia coli associated with diarrhoea in Malaysia containing plasmids showing homology with biotinylated Shiga-like toxin DNA gene probe. World Journal oj Microbiology and Biotechnology 12: 243-246.

Son, R., Rusul, G. and Karim, M. I. A. 1996b. Plasmid and antimicrobial resistance transfer from poultry to aquatic strains of Escherichia coli. Jurnal Veterinar Malaysia 8: 7-15.

Son, R., Rusul, G. and Karim, M. I. A. 1997a. Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia). Journal of Applied Microbiology 82: 240-244.

Son, R., Rusul, G., Karim, M. I. A. and Yusoff, K. 1997b. Detection of enterotoxigenic Escherichia coli associated with diarrhoea by biotinylated heat-stable enterotoxin (ST) DNA probe. Malaysian Applied Biology 26: 19-23.

Son, R., Sahilah, A. M., Rusul, G., Zainori, A., Morigaki, T., Asai, N., Kim, Y. B., Okuda, J. and Nishibuchi, M. 1998. Detection ol Escherichia coli O157:H7 in the beef marketed in Malaysia. Applied and Environmental Microbiology 64: 1153-1156.

Wilson, K. 1989. Preparation of Genomic DNA from Bacteria. In Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K., eds), pp. 2.4.1-2.4.5. John Wiley and Sons, New York.

Latest MABJ Issue

Vol 48(3) June 2019

Table of content

Latest news!

Malaysian Applied Biology is listed in the databases and indexed in Thomson Reuters Master Journal List, Myjurnal, Biosis, Zoological Records, UDLedge Life Science Index, CNKI, J-Gate and CABI.

Malaysian Applied Biology is indexed in Scopus since issue 41(1) 2012.

According to MyCite 2014 report, MABJ ranked 95 out of 142 Malaysian journals in terms of yearly impact factor.