Malaysian Applied Biology Journal

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Malays. Appl. Biol. (1999) 28(1&2): 43-48


Zuraimay, Z., Zamri, B., Sharifa, A.A. and Mohd Azman, A.B.

Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, 50300 Jalan Raja Muda, Kuala Lumpur, Malaysia


The aim is to study the effects of calcium antagonist (Diltiazem hydrochloride) on the changes in calcium and cholesterol levels of serum, bile, liver and gallbladder of male guinea pigs fed on high calcium diet for six weeks. The calcium levels determined by atomic absorption spectrophotometry were found to be increased in serum and gallbladder, whereas significantly raised in the liver and bile(P<0.01) after high calcium diet. Calcium antagonist showed increase levels of serum and biliary calcium if given alone or in combination with high calcium. In the liver and gallbladder, calcium levels were raised as compared to corresponding controls. The cholesterol level determined by gas chromatography showed that high calcium diet increased the serum cholesterol, significant reduction in gallbladder(p<0.05), but no significant difference in bile and liver after high calcium diet. Diltiazem as well as Diltiazem and high calcium combined group significantly increased the serum (p<0.05), increased in biliary cholesterol but significant reduction in liver (p<0.01) and gallbladder cholesterol (p<0.05) . Although there were increased levels of calcium and cholesterol in the bile, the stone detected was meagre. From this study, we found that calcium antagonist as well as high calcium diet increased serum calcium and cholesterol and that Diltiazem had more effects on the organ like liver and gallbladder than on serum and bile. Male hormone like testosterone and Diltiazem may have played a role in the prevention of stone as well as the drug. Since this is a preliminary study, further study is necessary to know more about the effects of calcium antagonist.

Key words: Calcium, Calcium Antagonist, Cholesterol, gallstone.


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